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Dental stem cell and dental tissue regeneration
Qiming Zhai, Zhiwei Dong, Wei Wang, Bei Li, Yan Jin
《医学前沿(英文)》 2019年 第13卷 第2期 页码 152-159 doi: 10.1007/s11684-018-0628-x
Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani
《化学科学与工程前沿(英文)》 2019年 第13卷 第1期 页码 108-119 doi: 10.1007/s11705-018-1742-7
Plants have been used for medicinal purposes for thousands of years but they are still finding new uses in modern times. For example, Elaeagnus angustifolia (EA) is a medicinal herb with antinociceptive, anti-inflammatory, antibacterial and antioxidant properties and it is widely used in the treatment of rheumatoid arthritis and osteoarthritis. EA extract was loaded onto poly(ϵ-caprolactone)-poly(ethylene glycol)-poly(ϵ-caprolactone) (PCL-PEG-PCL/EA) nanofibers and their potential applications for bone tissue engineering were studied. The morphology and chemical properties of the fibers were evaluated using Fourier transform infrared spectroscopy, field emission scanning electron microscopy, contact angle measurements and mechanical tests. All the samples had bead-free morphologies with average diameters ranging from 100 to 200 nm. The response of human cells to the PCL-PEG-PCL/EA nanofibers was evaluated using human dental pulp stem cells (hDPSCs). The hDPSCs had better adhesion and proliferation capacity on the EA loaded nanofibers than on the pristine PCL-PEG-PCL nanofibers. An alizarin red S assay and the alkaline phosphatase activity confirmed that the nanofibrous scaffolds induced osteoblastic performance in the hDPSCs. The quantitative real time polymerase chain reaction results confirmed that the EA loaded nanofibrous scaffolds had significantly upregulated gene expression correlating to osteogenic differentiation. These results suggest that PCL-PEG-PCL/EA nanofibers might have potential applications for bone tissue engineering.
关键词: Elaeagnus angustifolia scaffold electrospinning human dental pulp stem cell tissue engineering
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 306-315 doi: 10.1007/s11684-013-0279-x
Allogeneic hematopoietic stem cell transplantation (HSCT) is one of the most effective options for hematological malignancies, and human leukocyte antigen-partially matched related donors (PMRDs) are a valuable option for HSCT. Several protocols (with or without ex vivo T-cell depletion (TCD)) have been established worldwide. TCD including CD34+positive selection and CD3/CD19 depletion has successfully overcome the human leukocyte antigen disparity. However, TCD is associated with prolonged immune deficiencies, increased risks of infectious complications, and high transplantation-related mortality. PMRD HSCT without ex vivo TCD is well developed, and numerous patients have benefitted from it. Here, we review the literature on PMRD HSCT.
关键词: partially matched related donor hematopoietic stem cell transplantation allogeneic
Self-etching adhesives: possible new pulp capping agents to vital pulp therapy
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 77-79 doi: 10.1007/s11684-010-0104-8
Pulp capping is one of the solving for keeping vital pulp in the case of dentin caries, reversible pulpitis or traumatic pulp exposure. The presence of bacteria on the cavity walls or in the pulp was the major factor that leads to the failure of pulp capping. Traditional pulp capping agent, calcium hydroxide, may not prevent microleakage. Self-etching system is a newly developed adhesive system, which could provide less microleakage and would not break down or dissolve, preventing the oral fluids and bacteria from the pulp along the cavity wall. This may reduce such clinical problems as postoperative sensitivity, secondary caries and marginal discoloration. Researches showed that some kinds of self-etching adhesives induced the mild to moderate inflammatory pulp response, with negative bacterial staining. Inclusion of antibacterial components into self-etching system, such as 12- methacryloyloxydodecylpyridinium bromide (MDPB) may inhibit bacteria and provide better clinical effects. It is speculated that using the self-etching adhesive system containing the antibacterial agent, such as MDPB, to the dental pulp directly or indirectly, may inhibit bacteria after the placement of restoration as well as residual bacteria in the cavity.
关键词: dental pulp pulp capping self-etching adhesives antibacterial components
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
《医学前沿(英文)》 2007年 第1卷 第3期 页码 264-268 doi: 10.1007/s11684-007-0050-2
关键词: origin Little mesenchymal tissue cords/mesenchymal tissue CD34
人类诱导性多能干细胞稳定生产的最新进展 Review
Ikki Horiguchi, Masahiro Kino-oka
《工程(英文)》 2021年 第7卷 第2期 页码 144-152 doi: 10.1016/j.eng.2021.01.001
诱导性多能干细胞(iPSC)在许多方面,如再生医学和药物筛选方面,是理想的细胞来源,其开发前景广阔。但是,目前还没有一种有效的大规模生产系统来确保诱导性多能干细胞的稳定供应。本文综述了近年来为了实现诱导性多能干细胞的稳定生产所采取的方法。本文首先讨论了诱导性多能干细胞培养过程中需要控制的因素,如养分供应、废物清除和氧气供应。然后,介绍了基于黏附、悬浮和支架的诱导性多能干细胞培养系统的最新研究。本文还讨论了细胞培养阶段之后进行的过程,如灌装和冻存过程,由于细胞悬浮液在超低温保存培养基中存活率降低,所以其生产规模受到了限制。最后,我们总结了实现诱导性多能干细胞稳定生产的可能性,并强调了仍有待克服的局限性。本文表明为了获得最佳且稳定的大规模生产诱导性多能干细胞系统,运用多学科研究手段对研究影响细胞生长和质量的复杂因素是至关重要的。
The unregulated commercialization of stem cell treatments: a global perspective
Douglas Sipp
《医学前沿(英文)》 2011年 第5卷 第4期 页码 348-355 doi: 10.1007/s11684-011-0150-x
关键词: stem cell tourism medical ethics stem cell policy and regulation alternative medicine
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《医学前沿(英文)》 2015年 第9卷 第1期 页码 20-29 doi: 10.1007/s11684-014-0371-x
Ischemic stroke is a focal cerebral insult that often leads to many adverse neurological complications severely affecting the quality of life. The prevalence of stroke is increasing throughout the world, while the efficacy of current pharmacological therapies remains unclear. As a neuroregenerative therapy, the implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs) has shown great possibility to restore function after stroke. This review article provides an update role of hUC-MSCs implantation in the treatment of ischemic stroke. With the unique “immunosuppressive and immunoprivilege” property, hUC-MSCs are advised to be an important candidate for allogeneic cell treatment. Nevertheless, most of the treatments are still at primary stage and not clinically feasible at the current time. Several uncertain problems, such as culture conditions, allograft rejection, and potential tumorigenicity, are the choke points in this cellular therapy. More preclinical researches and clinical studies are needed before hUC-MSCs implantation can be used as a routinely applied clinical therapy.
关键词: cellular therapy transplantation human umbilical cord mesenchymal stem cells ischemic stroke
Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA
《化学科学与工程前沿(英文)》 2013年 第7卷 第4期 页码 482-489 doi: 10.1007/s11705-013-1342-5
关键词: pectic extracts antiproliferative activity breast cancer enzymatic treatment
The regulatory sciences for stem cell-based medicinal products
null
《医学前沿(英文)》 2014年 第8卷 第2期 页码 190-200 doi: 10.1007/s11684-014-0323-5
Over the past few years, several new achievements have been made from stem cell studies, many of which have moved up from preclinical stages to early, or from early to middle or late, stages thanks to relatively safe profile and preliminary evidence of effectiveness. Moreover, some stem cell-based products have been approved for marketing by different national regulatory authorities. However, many critical issues associated mainly with incomplete understanding of stem cell biology and the relevant risk factors, and lack of effective regulations still exist and need to be urgently addressed, especially in countries where establishment of appropriate regulatory system just commenced. More relevantly, the stem cell regulatory sciences need to be established or improved to more effectively evaluate quality, safety and efficacy of stem cell products, and for building up the appropriate regulatory framework. In this review, we summarize some new achievements in stem cell studies, especially the preclinical and clinical studies, the existing regulations, and the associated challenges, and we then propose some considerations for improving stem cell regulatory sciences with a goal of promoting the steadfast growth of the well-regulated stem cell therapies abreast of evolvement of stem cell sciences and technologies.
关键词: stem cell-based medicinal products (SCMPs) stem cell therapy (SCT) safety effectiveness standards guidelines regulatory science
Therapeutic potential of stem cell in liver regeneration
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 26-32 doi: 10.1007/s11684-011-0107-0
Liver transplantation is the only life-saving procedure for patients with end-stage liver disease. However, its potential benefits are hampered by many disadvantages, such as the relative shortage of donors, operative risks, and high costs. These issues have prompted the search for new alternative therapies for irreversible liver disease. Stem cell therapy, with the ability for self-renewal and potential for multilineage differentiation, is a promising alternative approach. Several studies have demonstrated that transplantation of hepatic stem/progenitor cells or hepatocyte-like cells derived from multipotent stem cells leads to donor cell-mediated repopulation of the liver and improved survival rates in experimental models of liver disease. However, a registered clinical application based on stem cell technology will take at least an additional 5 to 10 years because of some limitations; e.g. the lack of suitable cell sources and risk of teratoma formation. This review summarizes the general understanding of the therapeutic potentials of stem cells in liver disease, including the sources, mechanisms, and delivery methods of hepatic stem cells in liver regeneration, and discusses some challenges for their therapeutic application.
Stem cell niches and endogenous electric fields in tissue repair
null
《医学前沿(英文)》 2011年 第5卷 第1期 页码 40-44 doi: 10.1007/s11684-011-0108-z
Adult stem cells are responsible for homeostasis and repair of many tissues. Endogenous adult stem cells reside in certain regions of organs, known as the stem cell niche, which is recognized to have an important role in regulating tissue maintenance and repair. In wound healing and tissue repair, stem cells are mobilized and recruited to the site of wound, and participate in the repair process. Many regulatory factors are involved in the stem cell-based repair process, including stem cell niches and endogenous wound electric fields, which are present at wound tissues and proved to be important in guiding wound healing. Here we briefly review the role of stem cell niches and endogenous electric fields in tissue repair, and hypothesize that endogenous electric fields become part of stem cell niche in the wound site.
Transcriptomics and proteomics in stem cell research
null
《医学前沿(英文)》 2014年 第8卷 第4期 页码 433-444 doi: 10.1007/s11684-014-0336-0
Stem cells are capable of self-renewal and differentiation, and the processes regulating these events are among the most comprehensively investigated topics in life sciences. In particular, the molecular mechanisms of the self-renewal, proliferation, and differentiation of stem cells have been extensively examined. Multi-omics integrative analysis, such as transcriptomics combined with proteomics, is one of the most promising approaches to the systemic investigation of stem cell biology. We reviewed the available information on stem cells by examining published results using transcriptomic and proteomic characterization of the different stem cell processes. Comprehensive understanding of these important processes can only be achieved using a systemic methodology, and employing such method will strengthen the study on stem cell biology and promote the clinical applications of stem cells.
《医学前沿(英文)》 2023年 第17卷 第3期 页码 534-548 doi: 10.1007/s11684-022-0953-y
关键词: autoimmune hepatitis (AIH) concanavalin A (Con A) human menstrual blood-derived stem cells (MenSCs) apoptosis mitogen-activated protein kinase (MAPK)
《医学前沿(英文)》 2022年 第16卷 第2期 页码 227-239 doi: 10.1007/s11684-021-0896-8
关键词: cannabidiol depression radial neural stem cells neurogenesis
标题 作者 时间 类型 操作
Dental stem cell and dental tissue regeneration
Qiming Zhai, Zhiwei Dong, Wei Wang, Bei Li, Yan Jin
期刊论文
Effect of incorporating Elaeagnus angustifolia extract in PCL-PEG-PCL nanofibers for bone tissue engineering
Vahideh R. Hokmabad, Soodabeh Davaran, Marziyeh Aghazadeh, Effat Alizadeh, Roya Salehi, Ali Ramazani
期刊论文
Advancement of human leukocyte antigen-partially matched related hematopoietic stem cell transplantation
null
期刊论文
Immunohistochemical characterization of hepatic stem cell-related cells in developing human liver
XU Jun, HU Yong, WANG Jian, ZHANG Taiping, ZHOU Ji, YU Hongyu
期刊论文
Implantation of human umbilical cord mesenchymal stem cells for ischemic stroke: perspectives and challenges
null
期刊论文
Production of pectic extracts from sugar beet pulp with antiproliferative activity on a breast cancercell line
Jacqueline CONCHA, Caroline WEINSTEIN, María Elvira Zú?IGA
期刊论文
Human menstrual blood-derived stem cells alleviate autoimmune hepatitis via JNK/MAPK signaling pathway
期刊论文